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R6525

Sigma-Aldrich

Anti-phospho-Rsk1 (p90rsk) (pSer381) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-phospho-Rsk 1 (pSer381)

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About This Item

MDL number:
MFCD02097169
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: 1:2,000
immunoprecipitation (IP): 1:200
western blot (chemiluminescent): 1:1,000 using TPA-treated 293 cell extract

UniProt accession no.

Q15418

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RPS6KA1(6195)
mouse ... Rps6ka1(20111)
rat ... Rps6ka1(81771)

General description

Rsk1 is a ubiquitously expressed, mitogen-activated protein that belongs to the serine/threonine protein kinase family. It has a pivotal role in facilitating MEK-MAP kinase cell survival signaling and supressing pro-apoptotic function of BAD by its phosphorylation. Anti-phospho-Rsk1 (p90rsk) (pSer381) antibody can be used to detect Rsk1 when phosphorylated at phosphoserine 381. It can also be used in immunoprecipitation (diluted 1:200). Rabbit anti-phospho-Rsk1 (p90rsk) (pSer381) antibody reacts specifically with phosphorylated Rsk1 and not with nonphosphorylated Rsk1. The product has also specificity for Rsk1 of rat, mouse and human.

Immunogen

Synthetic phospho-Ser381 peptide corresponding to residues around Ser381 of human (p90rsk) conjugated to KLH.

Application

Anti-phospho-Rsk1 (p90rsk) (pSer381) antibody can be used in western blotting (diluted 1:1000) using TPA-treated 293 cell extract. It can also be used in immunocytochemistry (diluted 1:2000).

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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A Shimamura et al.
Current biology : CB, 10(3), 127-135 (2000-02-19)
Growth factors activate an array of cell survival signaling pathways. Mitogen-activated protein (MAP) kinases transduce signals emanating from their upstream activators MAP kinase kinases (MEKs). The MEK-MAP kinase signaling cassette is a key regulatory pathway promoting cell survival. The downstream

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