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Key Documents

R6018

Sigma-Aldrich

Ribonucleic acid, transfer, lysine specific from Escherichia coli

lyophilized powder

Synonym(s):

E. coli Lys tRNA, tRNA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200

grade

Molecular Biology

form

lyophilized powder

storage temp.

−20°C

General description

Transfer RNA is isolated from E. coli. This transfer RNA is specific for transfer of the amino acid lysine. The sequence and structure has been published previously.

Application

Suitable for use:
  • As a tRNA standard
  • As a substrate in tRNA synthatase assays
  • As a substrate in other reactions involving tRNAs

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Mridusmita Saikia et al.
RNA (New York, N.Y.), 16(7), 1317-1327 (2010-05-21)
The N(1)-methyl-Adenosine (m(1)A58) modification at the conserved nucleotide 58 in the TPsiC loop is present in most eukaryotic tRNAs. In yeast, m(1)A58 modification is essential for viability because it is required for the stability of the initiator-tRNA(Met). However, m(1)A58 modification
Tissue-specific differences in human transfer RNA expression.
Dittmar, K.A., et al.
PLoS Genetics, 2, 2107-2107 (2006)
V L Sharygin et al.
Radiatsionnaia biologiia, radioecologiia, 53(2), 151-169 (2013-06-22)
The responses of deoxyribonucleotide (dNTP), DNA and protein synthesis systems in blood-forming organs of animals (dogs, mice) as well as changes in Fe(3+)-transferrin (Fe(3+)-TF) and Cu(2+)-ceruloplasmin (Cu(2+)-CP) pools in blood to gamma-irradiation and the administration of radioprotectors have been studied.
Hanjoo Kim et al.
Nucleic acids research, 41(Web Server issue), W492-W498 (2013-06-14)
To facilitate the analysis of large-scale high-throughput capillary electrophoresis data, we previously proposed a suite of efficient analysis software named HiTRACE (High Throughput Robust Analysis of Capillary Electrophoresis). HiTRACE has been used extensively for quantitating data from RNA and DNA
North, south, or east? Blotting techniques.
M W Nicholas et al.
The Journal of investigative dermatology, 133(7), e10-e10 (2013-06-14)

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