R4530

Anti-Rab21 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-RAB21, member RAS oncogene family
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~25 kDa
• species reactivity: rat, mouse, human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using whole extract of human HeLa cells; indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 and rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin; western blot: 5-10 μg/mL using whole extract of human A431 cells
• UniProt accession no.: Q9UL25
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAB21(23011) ; mouse ... Rab21(216344) ; rat ... Rab21(299799)

Description

General description

Ras-related protein Rab-21 (Rab21), a close homolog of Rab5, is ubiquitously expressed in human tissues. It localizes mainly to the early endocytic pathway. Rab21 is mapped to human chromosome 12q21.1 It belongs to the Rab family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions, C-terminal hypervariable domains with a cysteine motif.

Specificity

Anti-Rab21 (C-terminal) recognizes human, rat, and mouse Rab21.

Immunogen

synthetic peptide corresponding to amino acid residues 203-216 of human RAB21, with an added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse.

Application

Anti-Rab21 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Ras-related protein Rab-21 (Rab21) and Rab5 are integrin-associated proteins, acting as positive regulators of integrin traffic. The association of Rab21 with integrins modulates cell adhesion and migration. It is also involved in endosome morphology and function. Rab21 is associated with squamous carcinoma and its expression is elevated in glioma.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extendedstorage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)