grade
Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
Storage temp.
−20°C
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Specificity
Recognition sequence: 5′-CCGC/GG-3′
Ligation and recutting results: After 2-10-fold Ksp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Ligation and recutting results: After 2-10-fold Ksp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Application
KspI is a restriction endonuclease that is used for molecular biology applications to cut DNA at the recognition sequence 5′-CCGC/GG-3′, generating DNA fragments with 3′-cohesive ends.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SL (B3782).
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% gelatine (v/v), 0.2% polydocanol (v/v) at 4°C
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O A Oyarzabal et al.
Journal of applied microbiology, 105(3), 800-812 (2008-04-16)
Thirty Campylobacter jejuni strains isolated from fecal samples (n = 94; 32%) from 13 positive farms (n = 17; 76%) from commercial broiler chickens in Puerto Rico were analysed by molecular methods. Isolates were identified with multiplex polymerase chain reaction
KspI, a novel isoschizomer of SacII from Kluyvera species recognizing 5'-CCGC/GG-3'.
B J Bolton et al.
Nucleic acids research, 17(22), 9476-9476 (1989-11-25)
Leonardo J van Zyl et al.
Applied and environmental microbiology, 74(18), 5686-5694 (2008-07-29)
Acidithiobacillus caldus is a moderately thermophilic, acidophilic bacterium that has been reported to be the dominant sulfur oxidizer in stirred-tank processes used to treat gold-bearing arsenopyrite ores. It is also widely distributed in heap reactors used for the extraction of
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Gajendradhar R Dwivedi et al.
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against
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