R2780
Anti-RAE1 (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-MRNP41, Anti-RNA export 1, Anti-mRNA-binding protein, 41 kDa
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 43 kDa
species reactivity
human, rat, mouse (predicted)
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using HEK-293T and RIN5 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAE1(8480)
mouse ... Rae1(66679)
rat ... Rae1(362281)
General description
Ribonucleic acid export 1 (RAE1/RNA export 1) also known as mRNA-binding protein.is a constituent of the mRNA export machinery. RAE1 has three protein/protein domains named β-transducin/WD40 motifs. The human RAE1 has a high homology to S.pombe Rae1 and Gle2 protein in S.cerevisiae. RAE1 gene is located on human chromosome 20q13.32.
Specificity
Anti-RAE1 (N-terminal) specifically recognizes human and rat RAE1 (43 kDa).
Immunogen
synthetic peptide corresponding to amino acids 18-35 of human RAE1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence in mouse and rat differs by one amino acid.
application
Anti-RAE1 (N-terminal) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Ribonucleic acid export 1 (RAE1/RNA export 1) participates in bipolar spindle formation through its interaction with nuclear mitotic apparatus protein (NuMA) and its binding to microtubules. RAE1 and nuclear pore complex protein (Nup98) form a complex with cdh1 and anaphase-promoting complex (APC) in early mitosis and specifically inhibit APC (Cdh1)-mediated ubiquitination of securin. It acts as a mitotic checkpoint regulator. It may act as a prognostic marker in breast cancers. Mutations in RAE1 leads in the accumulation of poly(A)-containing mRNA in the nucleus. It plays a key role in nucleoporin 98?kDa (NUP98)-mediated tumorigenesis in acute myeloid leukemia. Overexpression of this gene is observed in lung cancer.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to onemonth. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Localization of a novel autosomal recessive nonsyndromic hearing impairment locus DFNB65 to chromosome 20q13. 2-q13. 32
Tariq A, et al.
Journal of Molecular Medicine, 84(6), 484-490 (2006)
The mitotic checkpoint regulator RAE1 induces aggressive breast cancer cell phenotypes by mediating epithelial-mesenchymal transition
Oh JH, et al.
Scientific Reports, 7, 42256-42256 (2017)
RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains
Pritchard CE, et al.
The Journal of Cell Biology, 145(2), 237-254 (1999)
Karthik B Jeganathan et al.
Nature, 438(7070), 1036-1039 (2005-12-16)
Cdc20 and Cdh1 are the activating subunits of the anaphase-promoting complex (APC), an E3 ubiquitin ligase that drives cells into anaphase by inducing degradation of cyclin B and the anaphase inhibitor securin. To prevent chromosome missegregation, APC activity directed against
Yi Ren et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(23), 10406-10411 (2010-05-26)
The export of mRNAs is a multistep process, involving the packaging of mRNAs into messenger ribonucleoprotein particles (mRNPs), their transport through nuclear pore complexes, and mRNP remodeling events prior to translation. Ribonucleic acid export 1 (Rae1) and Nup98 are evolutionarily
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