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Key Documents

R2775

Sigma-Aldrich

Monoclonal Anti-RasGAP antibody produced in mouse

clone B4F8, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal Anti-Gap, Monoclonal Anti-Ras GTPase-activating protein, Monoclonal Anti-Rasa1

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About This Item

MDL number:
UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B4F8, monoclonal

form

buffered aqueous glycerol solution

species reactivity

hamster, rat, mouse, bovine, human

technique(s)

immunocytochemistry: 10 μg/mL using A431 cells fixed with ethanol:acetic acid (95:5)
immunoprecipitation (IP): 2.5 μg using 500 μg of A431 cell lysate
western blot: 0.5-2 μg/mL using 20 μg of A431 cell lysate

isotype

IgG

UniProt accession no.

shipped in

wet ice

Storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RASA1(5921)
mouse ... Rasa1(218397)
rat ... Rasa1(25676)

Immunogen

full-length recombinant human GAP expressed in Sf9 insect cells.

Physical form

Solution in 0.07 M Tris-glycine, pH 7.4, containing 0.105 M sodium chloride, 30% glycerol, and 0.035% sodium azide.

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K Y Ng et al.
Oncogene, 34(20), 2650-2659 (2014-07-22)
The Crk SH2/SH3 adaptor and the Abl nonreceptor tyrosine kinase were first identified as oncoproteins, and both can induce tumorigenesis when overexpressed or mutationally activated. We previously reported the surprising finding that inhibition or knockdown of Abl family kinases enhanced

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