R2645
Ribonucleic acid, transfer, valine specific from Escherichia coli
lyophilized powder
Synonym(s):
tRNA
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About This Item
form
lyophilized powder
storage temp.
−20°C
Application
Ribonucleic acid, transfer, valine-specific may be used as a substrate to study the specifity and kinetics of natural, mutated and chemically engineered valine-specific E. coli aminoacyl-tRNA synthetase(s).
Quality
Acceptor Activity: approx. 1300 picomoles valine per A260 unit (not determined by Sigma)
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Keith D Tardif et al.
RNA (New York, N.Y.), 10(3), 493-503 (2004-02-19)
To correct misactivation and misacylation errors, Escherichia coli valyl-tRNA synthetase (ValRS) catalyzes a tRNA(Val)-dependent editing reaction at a site distinct from its aminoacylation site. Here we examined the effects of replacing the conserved 3'-adenosine of tRNA(Val) with nucleoside analogs, to
Codjo Hountondji et al.
Biochemistry, 41(50), 14856-14865 (2002-12-12)
Valyl-tRNA synthetase (ValRS) from Escherichia coli undergoes covalent valylation by a donor valyl adenylate synthesized by the enzyme itself. ValRS could also be modified, although to a lesser extent, by the noncognate isosteric substrate L-threonine from a donor threonyl adenylate
C Hountondji et al.
European journal of biochemistry, 267(15), 4789-4798 (2000-07-21)
Bromomethyl ketone derivatives of L-valine (VBMK), L-isoleucine (IBMK), L-norleucine (NleBMK) and L-phenylalanine (FBMK) were synthesized. These reagents were used for qualitative comparative labeling of Escherichia coli valyl-tRNA synthetase (ValRS), an enzyme with Val/Ile editing activity, in order to identify the
K D Tardif et al.
Biochemistry, 40(27), 8118-8125 (2001-07-04)
Valyl-tRNA synthetase (ValRS) has difficulty discriminating between its cognate amino acid, valine, and structurally similar amino acids. To minimize translational errors, the enzyme catalyzes a tRNA-dependent editing reaction that prevents accumulation of misacylated tRNA(Val). Editing occurs with threonine, alanine, serine
SnapShot: force spectroscopy and single-molecule manipulation.
Yeonee Seol et al.
Cell, 153(5), 1168-1168 (2013-05-28)
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