R1155

Anti-AtRabA4 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-AtGB3, Anti-AtRab11G, Anti-AtRabA4b
• MDL number: MFCD09753849
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: Arabidopsis thaliana
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole cell extract of E. coli expressing GST-AtRabA4b fusion protein
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Arabidopsis thaliana ... RABA4B(830160)

Description

General description

The Arabidopsis thaliana Rab GTPase (RabA4b) is a Rab GTPase, that is expressed in various plant organs. In A. thaliana, RabA4b is ubiquitously expressed, mainly located at an inverted cone-shaped area in the apical region in the root hairs. The Arabidopsis genome contains a total of 93 small GTPase genes, including 57 members of the Rab family. Arabidopsis thaliana Rab GTPase RabA4b (AtRabA4b, AtGB3, AtRab11G) is highly similar to AtRab11.

Specificity

Anti-AtRabA4bspecifically recognizes recombinant AtRabA4b fusion protein.

Immunogen

synthetic peptide corresponding to amino acids 201-219 located near the C-terminus of Arabidopsis thaliana AtRabA4b fusion protein.

Application

Anti-AtRabA4 antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

The Arabidopsis thaliana Rab GTPase (RabA4b) can control membrane trafficking steps involved in the polarized deposition of cell wall components in tip-growing root hair cells. AtRabA4b can label the trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing root hair cells of A. thaliana.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.