R1030

Monoclonal Anti-RAP1 A/B antibody produced in mouse

~1 mg/mL, clone R116, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-KREV-1, Anti-KREV1, Anti-RAP1, Anti-SMGP21
• MDL number: MFCD01634083
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: R116, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~21 kDa (additional band at 70 kDa may be seen in some preparations)
• species reactivity: hamster, human
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using total cell extract of CHO cells
• isotype: IgM
• UniProt accession no.: P61224; P62834
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAP1A(5906) , RAP1B(5908)

Description

General description

Monoclonal Anti-RAP1 A/B (mouse IgM isotype) is derived from the hybridoma R116 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. Ras-related protein 1A (RAP1A/Krev-1) belongs to the Ras superfamily of small GTPases. RAP1 gene is mapped to human chromosome 1p13.3

Specificity

Monoclonal Anti-RAP1 A/B recognizes human and hamster RAP1 A/B (~21 kDa).

Immunogen

synthetic peptide corresponding to amino acids 26-40 of human RAP1. The antibody recognizes human and hamster RAP1 A/B.

Application

Monoclonal Anti-RAP1 A/B antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Ras-related protein 1A (RAP1A), a novel oncoprotein in human malignancies acts as an important modulator in T cell response. RAP1A also participates in bacterial infection, cell dynamics and osteoblast differentiation. It may serve as a Ras antagonist and is a tumor suppressor gene. RAP1A is activated by growth factors, neurotransmitters and cytokines. Several second messengers, such as cyclic adenosine monophosphate (cAMP), diacylglycerol and calcium may serve as the mediators of this activation. In many cells, active RAP1 changes its cellular location from endosomal membranes to the plasma membrane. RAP1 binds competitively to RAS-GTPase activating protein (RASA) thus interfering with its RAS effectors functions. Ectopically expressed RAP1 is capable of interacting with the RAS effector (RAF1), which may lead to the inhibition of extra-cellular-signal-regulated kinase (ERK) activation. RAP1 can also mediate cyclic adenosine monophosphate (cAMP)-induced ERK activation.

Target description

Monoclonal Anti-RAP1 A/B recognizes human and hamster RAP1 A/B (~21 kDa). Ras-related proteins are p21 superfamily of proteins involved in cell growth and differentiation. The p21 protein RAP1, expressed in cells as one of two isoforms (RAP1A and RAP1B),

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)