R0258
Ribonucleic acid, transfer, tyrosine-specific I from Escherichia coli
lyophilized powder
Synonym(s):
tRNA
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
form
lyophilized powder
storage temp.
−20°C
Application
Ribonucleic acid, transfer, tyrosine-specific I may be used as a substrate to study the specifity and kinetics of natural, mutated and chemically engineered tyrosine-specific E. coli aminoacyl-tRNA synthetase(s).
Quality
Acceptor Activity: ≥900picomoles tyrosine per A260 unit (not determined by Sigma)
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Kenji Oki et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13298-13303 (2008-09-04)
To guarantee specific tRNA and amino acid pairing, several aminoacyl-tRNA synthetases correct aminoacylation errors by deacylating or "editing" misaminoacylated tRNA. A previously developed variant of Escherichia coli tyrosyl-tRNA synthetase (iodoTyrRS) esterifies or "charges" tRNA(Tyr) with a nonnatural amino acid, 3-iodo-l-tyrosine
Fumie Iraha et al.
Nucleic acids research, 38(11), 3682-3691 (2010-02-18)
Non-natural amino acids have been genetically encoded in living cells, using aminoacyl-tRNA synthetase-tRNA pairs orthogonal to the host translation system. In the present study, we engineered Escherichia coli cells with a translation system orthogonal to the E. coli tyrosyl-tRNA synthetase
Kensaku Sakamoto et al.
Nucleic acids research, 30(21), 4692-4699 (2002-11-01)
A suppressor tRNA(Tyr) and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-L-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNA(Tyr) gene was mutated, at three positions in the D arm, to generate the internal promoter
Sara F Hansson et al.
PloS one, 13(4), e0196601-e0196601 (2018-04-28)
Beta cell dysfunction accompanies and drives the progression of type 2 diabetes mellitus (T2D), but there are few clinical biomarkers available to assess islet cell stress in humans. Secretagogin, a protein enriched in pancreatic islets, demonstrates protective effects on beta
Charles E Melançon et al.
Bioorganic & medicinal chemistry letters, 19(14), 3845-3847 (2009-04-29)
We have developed a rapid, straightforward, one plasmid dual positive/negative selection system for the evolution of aminoacyl-tRNA synthetases with altered specificities in Escherichia coli. This system utilizes an amber stop codon containing chloramphenicol acetyltransferase/uracil phosphoribosyltransferase fusion gene. We demonstrate the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service