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Key Documents

Q0134

Sigma-Aldrich

Q-Ceramic HyperD® 20

20 μm mean particle size

Synonym(s):

HyperD® ceramic ion exchangers

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About This Item

UNSPSC Code:
23201100

mean particle size

20 μm

capacity

>0.20 meq/mL

storage temp.

2-8°C

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Application

Ceramic HyperD media are used in protein chromatography and affinity chromatography. Ceramic HyperD media have been used in the development of a capture step of a human recombinant F(ab′)(2) produced and expressed in baculovirus-infected cells and to identify whey proteins as a model system for chromatographic separation of proteins.

Features and Benefits

HyperD media are highly porous, rigid ceramic beads filled with a functionalized hydrophilic gel. The rigid bead allows extremely high linear flow rates without bed compression. The hydrogel exchanges throughout its volume, not just on the surface. And the media do not change volume due to changes in pH or ionic strength.
The supports are stable in commonly used solvents and alkaline or acid environments. Supplied as aqueous suspensions in 1 M NaCl with 20% ethanol to inhibit bacterial growth.

Legal Information

HyperD is a registered trademark of Pall Corporation

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J S Aarnikunnas et al.
Applied and environmental microbiology, 72(1), 368-377 (2006-01-05)
The Pantoea ananatis ATCC 43072 mutant strain is capable of growing with xylitol as the sole carbon source. The xylitol-4-dehydrogenase (XDH) catalyzing the oxidation of xylitol to L-xylulose was isolated from the cell extract of this strain. The N-terminal amino
Mashkoor Alam et al.
Journal of separation science, 33(12), 1723-1729 (2010-05-22)
Working with biological fluids poses a challenge of visualizing proteins present in lower concentrations. This study describes a batch-mode chromatographic method for the fractionation of human amniotic fluid (AF). This method is easy to use with minimal sample quantity, resin
Arne Staby et al.
Journal of chromatography. A, 1164(1-2), 82-94 (2007-07-31)
A comparative study on weak anion exchangers was performed to investigate the pH dependence, binding strength, particle size distribution, and static and dynamic capacity of the chromatographic resins. The resins tested included: DEAE Sepharose FF, Poros 50 D, Fractogel EMD
Jochen Urthaler et al.
Journal of chromatography. A, 1065(1), 93-106 (2005-03-24)
The demand of high-purity plasmid DNA (pDNA) for gene-therapy and genetic vaccination is still increasing. For the large scale production of pharmaceutical grade plasmids generic and economic purification processes are needed. Most of the current processes for pDNA production use
Towards the design of a scalable and commercially viable technique for plasmid purification using a methacrylate monolithic stationary phase.
Danquah, M.K., and Forde, G.M.
Journal of Chemical Technology and Biotechnology, 82, 752-757 (2007)

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