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Sigma-Aldrich

GlycoProfile™ I, In-Gel Deglycosylation Kit

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1 KIT
$657.00

$657.00

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1 KIT
$657.00

About This Item

EC Number:
232-615-7
UNSPSC Code:
12352200
NACRES:
NA.32

$657.00

Check Cart for Availability
Request a Bulk Order

shelf life

≥1 yr at 2‑8 °C

Quality Level

200

storage temp.

2-8°C

Application

The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Principle

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Quantity

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Acetonitrile, ≥99.93%, BioSyn® 50 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 USDS
  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgSDS
  • Trypsin Solubilization Reagent
  • I0408Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiaeSDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

35.6 °F

flash_point_c

2.0 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLBV6157
037K6810
016K6837
065K6251
023K9176

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Articles

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Deglycosylation Kits

Find deglycosylation kits for your research. Review the different features of deglycosylation kits for glycoproteins, enzymatic proteins, and native proteins.

O-Linked Glycan Strategies

Learn about O-linked glycan strategies, O-glycosidase actions, how to remove sialic acid residues, β-Elimination, and O-glycan modifications.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

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