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PIC0006

Sigma-Aldrich

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, Non-freezing solution

Synonym(s):

Protease inhibitor cocktail

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.28

product name

ReadyShield® Protease Inhibitor Cocktail, for use in tissue culture media, Non-freezing solution

description

Tissue Culture Media

form

ready-to-use solution

quality

200

packaging

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

storage temp.

−20°C

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General description

This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Inhibits serine, cysteine, aspartic proteases and aminopeptidases.
  • Tailored for use in tissue culture media.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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