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shipped in
dry ice
storage temp.
−20°C
Application
pT7-FLAGa-4 is a 6451 bp Escherichia coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAGÒ fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORF-FLAG fusion constructs.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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F W Studier et al.
Journal of molecular biology, 189(1), 113-130 (1986-05-05)
A gene expression system based on bacteriophage T7 RNA polymerase has been developed. T7 RNA polymerase is highly selective for its own promoters, which do not occur naturally in Escherichia coli. A relatively small amount of T7 RNA polymerase provided
A H Rosenberg et al.
Gene, 56(1), 125-135 (1987-01-01)
Plasmid vectors are described that allow cloning of target DNAs at sites where they will be minimally transcribed by Escherichia coli RNA polymerase but selectively and actively transcribed by T7 RNA polymerase, in vitro or in E. coli cells. Transcription
Articles
Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.
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