P9529

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

lyophilized powder

• Synonym(s): 3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE
• CAS Number: 9040-59-9
• Enzyme Commission number: 3.1.4.17 (BRENDA, IUBMB)
• EC Number: 232-920-5
• MDL number: MFCD00131864
• UNSPSC Code: 12352202

Properties

• biological source: bovine brain
• form: lyophilized powder
• specific activity: 1-5 units/mg protein (without added calmodulin)
• mol wt: ~60 kDa
• composition: Protein, 4.0-6.0% Lowry
• UniProt accession no.: P14100
• foreign activity: 2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%; 5′-ATPase ≤0.2%; inorganic pyrophosphatase ≤0.8%
• storage temp.: −20°C
• Gene Information: cow ... PDE1A(281969)

Description

General description

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca²⁺ second-messenger systems.[1]

Application

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin[2] and in in vitro adenylate cyclase assay.[3]

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Physical form

Lyophilized powder containing Tris-HCl buffer salts and lactose

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

Analysis Note

Protein determined by Warburg-Christian

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)