P9498

Monoclonal Anti-PIAS-x antibody produced in mouse

clone PIASX-116, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Protein Inhibitor of Activator STAT
• MDL number: MFCD08705595
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PIASX-116, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~75 kDa
• species reactivity: human
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using proteasome fraction II of HeLa cells
• isotype: IgG1
• UniProt accession no.: O75928
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIAS2(9063)

Description

General description

Monoclonal Anti-PIAS-x (mouse IgG1isotype) is derived from the PIASX-116 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. Protein inhibitor of activated STAT 2 (PIAS2/PIAS-x), a small ubiquitin-like modifier (SUMO) E3 ligase contains a zinc binding motif and a highly acidic region. This gene is located on human chromosome 18q21.1.

Specificity

Monoclonal Anti-PIAS-x recognizes human PIAS-x.

Immunogen

peptide corresponding to amino acids 26-40 of human PIAS-x.

Application

Monoclonal Anti-PIAS-x antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunoblotting

Biochem/physiol Actions

Protein inhibitor of activated STAT2 (PIAS2) plays a key role in SUMO modification (SUMOylation) of several host and viral proteins. PIAS-x acts as a transcriptional co-repressor of STAT4. It may participate in controlling the chromatin structure. Upregulation or suppression of this gene can control the stability of the hepatitis C virus (HCV) core, NS3 and NS5A proteins. Hence PIAS-x is considered as the restriction factor for HCV replication.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recom-mended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.