P9371

Monoclonal Anti-PINCH-1 antibody produced in mouse

clone PINCH-N173, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD06798713
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PINCH-N173, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~37 kDa
• species reactivity: monkey, canine, mouse, human, rat, bovine
• technique(s): indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of 293T cells
• isotype: IgM
• UniProt accession no.: P48059
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LIMS1(3987) ; mouse ... Lims1(110829) ; rat ... Gcc2(309798)

Description

General description

Monoclonal Anti-PINCH-1 (mouse IgM isotype) is derived from the hybridoma PINCH-N173 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. PINCH1 (particularly interesting new Cys?His protein 1) also named as LIM zinc finger domain containing 1 (LIMS1), is a member of the LIM family. This gene is located on human chromosome 2q12.3. Human PINCH-1 and 2 are encoded by two different genes that are 82% identical in their amino acid sequence.

Specificity

Monoclonal Anti-PINCH-1 recognizes human, monkey, bovine, canine, rat, and mouse PINCH-1 (~ 37 kDa).

Immunogen

peptide corresponding to amino acids 14-31 of human PINCH-1, conjugated to KLH.

Application

Monoclonal Anti-PINCH-1 antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Particularly interesting new Cys−His protein 1(PINCH1) plays an important role in the cellular cardiomyocyte function development. Removal of PINCH1 can impair ischemic damage. PINCH1 can also serve as a stress sensors. Monoclonal antibodies specific for PINCH-1 are an important tool for studying intracellular signaling involved in cell adhesion.

Target description

PINCH-1 gene encodes a protein that is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable