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P9172

Sigma-Aldrich

Pyronin Y

for NA electrophoresis

Synonym(s):

Pyronin Y, Pyronin G, Pyronin J

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$261.00

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1 G
$70.10
5 G
$261.00

About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12171500
PubChem Substance ID:
57654569
NACRES:
NA.52

$70.10

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grade

Molecular Biology

Quality Level

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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Application

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)[1]
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)[2]
  • staining of CD34+ cells[3]

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells.[4] In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000256525
SHBP6260
SHBP3367
SHBN0822
MKCB9857

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Transcriptional profiling of mammary gland side population cells
Behbod F, et al.
Stem Cells, 24(4), 1065-1074 (2006)
Cristina Mesa-Núñez et al.
Stem cell research & therapy, 11(1), 164-164 (2020-04-30)
CD18 is the common beta subunit of β2 integrins, which are expressed on hematopoietic cells. β2 integrins are essential for cell adhesion and leukocyte trafficking. Here we have analyzed the expression of CD18 in different subsets of human hematopoietic stem
Green, F.J., ed.
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 601-602 (1990)
Staining of RNA after polyacrylamide gel electrophoresis.
K Marcinka
Methods in cell biology, 10, 49-68 (1975-01-01)
Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres
Wang J, et al.
Blood, 124(22), 3237-3240 (2014)
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