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P8248

Sigma-Aldrich

Monoclonal Anti-Profilin antibody produced in mouse

~2 mg/mL, clone 2B8 (MAbPRF45a), purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
MFCD01865559
UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2B8 (MAbPRF45a), monoclonal

form

buffered aqueous solution

mol wt

antigen ~14 kDa

species reactivity

Arabidopsis thaliana

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using total extracts of Arabidopsis flower

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

Gene Information

Arabidopsis thaliana ... PRF4(829055)

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General description

Profilins are highly conserved pan-allergens that occur in plant foods, pollen and latex. Profilins have five distinct isoforms (PRF1-5) that bind with actin filaments to regulate cytoskeletal organization. PRF4 and PRF5 are cytoplasmic proteins that are expressed in mature pollens . Monoclonal Anti-Profilin antibody is specific for Arabidopsis pollen profilin (PRF4 and PRF5). The antibody detects tobacco and maize profilin (approx. 14 kDa), but does not react with constitutive specific profilins (PRF1, 2, and 3).

Immunogen

profilin PRF4 protein.

Application

Monoclonal Anti-Profilin antibody is suitable for use in western blot (1-2 μg/mL using total extracts of Arabidopsis flower), indirect ELISA and immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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Muthugapatti K Kandasamy et al.
Cell motility and the cytoskeleton, 52(1), 22-32 (2002-04-27)
Profilin is a low-molecular weight, actin monomer-binding protein that regulates the organization of actin cytoskeleton in eukaryotes, including higher plants. Unlike the simple human or yeast systems, the model plant Arabidopsis has an ancient and highly divergent multi-gene family encoding
Alexandra Santos et al.
International archives of allergy and immunology, 155(3), 191-204 (2011-02-05)
Profilins are ubiquitous proteins, present in all eukaryotic cells and identified as allergens in pollen, latex and plant foods. The highly conserved structure justifies the cross-reactive nature of IgE antibodies against plant profilins and their designation as pan-allergens. Primary sensitization

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