P7996
Anti-phospho-PDGF Rα (pTyr762) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-phospho-Platelet Derived Growth Factor Receptor α
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, mouse, rat
technique(s)
western blot: 0.35-1.0 μg/mL using using NIH3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDGFA(5154)
mouse ... Pdgfa(18590)
rat ... Pdgfa(25266)
General description
Platelet-derived growth factor (PDGF) is a serum growth factor purified from human platelets. The members of PDGF are homodimers of A-, B-, C- and –D polypeptide chains and an AB heterodimer. PDGF is a mitogen and stimulates the proliferation of cells of connective tissue and glial cells, survival, differentiation, chemotaxis and wound healing. The cellular effects of PDGF isoforms are mediated by binding to receptors PDGF Rα and PDGF Rβ. Phosphorylation of PDGF Rα at tyrosine residues leads to activation of downstream signaling via PI3K, STAT and Ras/MAPK. Overexpression of PDGF isoforms has been observed in various malignancies including gliomas, osetosarcoma, chronic myelomonocytic leukemia and non-small-cell lung cancers
Auto-phosphorylation of PDGF Rα at Tyr762 aids in binding Crk proteins. The antibody detects mouse PDGF Rα. It may cross react with human, rat (100% homologous) and frog (83%) PDGF Rα. It does not react with PDGF Rβ (50%).
Auto-phosphorylation of PDGF Rα at Tyr762 aids in binding Crk proteins. The antibody detects mouse PDGF Rα. It may cross react with human, rat (100% homologous) and frog (83%) PDGF Rα. It does not react with PDGF Rβ (50%).
Immunogen
synthetic phosphopeptide derived from the region of human PDGF receptor α containing tyrosine 762.
Application
Anti-phospho PDGF Rα may be used for immunoblotting at a concentration of 0.35 to 1 μg/ml using NIH3Ts cells.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free), and 0.05% sodium azide.
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Inga Nazarenko et al.
Upsala journal of medical sciences, 117(2), 99-112 (2012-04-19)
The family of platelet-derived growth factors (PDGFs) plays a number of critical roles in normal embryonic development, cellular differentiation, and response to tissue damage. Not surprisingly, as it is a multi-faceted regulatory system, numerous pathological conditions are associated with aberrant
PDGF in gliomas: more than just a growth factor?
Lindberg N and Holland EC
Upsala Journal of Medical Sciences, 117, 92-98 (2010)
A Smits et al.
The American journal of pathology, 140(3), 639-648 (1992-03-01)
Platelet-derived growth factor (PDGF) is known to stimulate the proliferation of connective tissue-derived cells in vitro. Less is known about its functions in vivo, and the role of PDGF in the development of human tumors has not been clarified. The
Daniel F Bowen-Pope et al.
Arteriosclerosis, thrombosis, and vascular biology, 31(11), 2397-2401 (2011-10-21)
A growth-promoting activity released from activated platelets, the platelet-derived growth factor, was discovered and characterized while the cellular and molecular mechanisms underlying the formation of the lesions of atherosclerosis were being investigated. This review provides a personal account of the
Carl-Henrik Heldin
Upsala journal of medical sciences, 117(2), 83-91 (2012-04-20)
Platelet-derived growth factor (PDGF) isoforms are important mitogens for different types of mesenchymal cells, which have important functions during the embryonal development and in the adult during wound healing and tissue homeostasis. In tumors, PDGF isoforms are often over-expressed and
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