P7348
T7 Phage Promoter Primer Set
Synonym(s):
5′-TAA TAC GAC TCA CTA TAG GG-3′
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About This Item
UNSPSC Code:
41106305
Recommended Products
storage temp.
2-8°C
Application
T7 Phage Promoter Primer Set is a single-stranded oligonucleotide with 5′-hydroxyl and 3′-hydroxyl ends and a selection of four fluorescent lables for use in polymerase chain reaction protocols (PCR). Functionally tested for use in fluorescence-detection automated sequencing.
Packaging
Packaged in amber tubes to protect from light.
Components
Set of four vials of primer, each individually labeled at the 5′-end with:
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl
Analysis Note
Tested for purity with HPLC, PAGE, and OD.
Storage Class
10 - Combustible liquids
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Yajing Zhou et al.
PloS one, 6(7), e22224-e22224 (2011-07-27)
Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from
High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit 7-Unit 7 (2011)
Jacquelina Williams-Woods et al.
Journal of virological methods, 178(1-2), 253-257 (2011-10-04)
Human norovirus (HuNoV) and hepatitis A (HAV) are recognized as leading causes of non-bacterial foodborne associated illnesses in the United States. DNA sequencing is generally considered the standard for accurate viral genotyping in support of epidemiological investigations. Due to the
K A Abd-Elsalam et al.
Genetics and molecular research : GMR, 9(4), 2016-2024 (2010-10-20)
Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed
Angela Capece et al.
International journal of food microbiology, 144(1), 187-192 (2010-10-12)
The present research studied Saccharomyces cerevisiae yeasts isolated from Nero d'Avola grapes, collected in different areas of the Sicily region. RAPD-PCR analysis with M13 primer was used for preliminary discrimination among 341 S. cerevisiae isolates. Inoculated fermentations with S. cerevisiae
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