P6498
Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody produced in rat
~1.5 mg/mL, clone 6F9, purified immunoglobulin, buffered aqueous solution
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F9, monoclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
rat, frog, human, mouse
concentration
~1.5 mg/mL
technique(s)
direct ELISA: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cells extract.
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... PPP2R1A(5518)
mouse ... Ppp2ca(19052)
rat ... Ppp2ca(24672)
General description
PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates localization of Sgo1 at centromeres, Ras-1 activation, and chromosome segregation . This enzyme primarily comprises of three components; structural subunit A, regulatory subunit B and catalytic subunit C. The scaffolding subunit A has 2 isoforms (α and β) that have been implicated in tumor suppression
Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody is specific for human, rat, mouse, pig and xenopus PP2Aα (approx. 65 kDa).
Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody is specific for human, rat, mouse, pig and xenopus PP2Aα (approx. 65 kDa).
Immunogen
11 amino acid peptide, corresponding to the N terminus of the Aα subunit, coupled to bovine serum albumin.
This sequence is identical in human, rat, mouse and frog PP2Aα proteins.
Application
Monoclonal Anti-Protein Phosphatase 2Aα (PP2Aα) antibody is suitable for use in western blot (1:200) and immunohistochemistry (1:10) using pig myocardial tissue . The antibody can also be used for direct ELISA, and immunoprecipitation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Anna A Sablina et al.
Cell adhesion & migration, 1(3), 140-141 (2007-07-01)
The protein phosphatase 2A (PP2A) family of heterotrimeric serine-threonine phosphatases participates in human cell transformation. Each functional PP2A complex contains one structural A subunit (A alpha or A beta), and mutations of both are found to occur at low frequency
Andreas Totzeck et al.
American journal of physiology. Heart and circulatory physiology, 295(5), H2106-H2112 (2008-10-07)
Cardiac connexin 43 (Cx43) is involved in infarct propagation, and the uncoupling of Cx43-formed channels reduces infarct size. Cx43-formed channels open upon Cx43 dephosphorylation, and ischemic preconditioning (IP) prevents the ischemia-induced Cx43 dephosphorylation. In addition to the sarcolemma, Cx43 is
He-Ge Chen et al.
PloS one, 4(9), e7019-e7019 (2009-09-15)
Protein phosphatases-2A (PP-2A) is a major serine/threonine phosphatase and accounts for more than 50% serine/threonine phosphatase activity in eukaryotes. The holoenzyme of PP-2A consists of the scaffold A subunit, the catalytic C subunit and the regulatory B subunit. The scaffold
D Abraham et al.
The Journal of biological chemistry, 275(29), 22300-22304 (2000-05-10)
The Raf-1 kinase plays a key role in relaying proliferation signals elicited by mitogens or oncogenes. Raf-1 is regulated by complex and incompletely understood mechanisms including phosphorylation. A number of studies have indicated that phosphorylation of serines 259 and 621
Satoshi Ugi et al.
Molecular and cellular biology, 22(7), 2375-2387 (2002-03-09)
Protein phosphatase 2A (PP2A) is a multimeric serine/threonine phosphatase that carries out multiple functions. Although numerous observations suggest that PP2A plays a major role in downregulation of the mitogen-activated protein (MAP) kinase pathway, the precise mechanisms are unknown. To clarify
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