P6495

Anti-PRMT6 (SE-15) antibody produced in rabbit

~0.9 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Protein Arginine Methyl Transferase 6
• MDL number: MFCD05665251
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~0.9 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5 μg using extracts of PRMT6 transfected 293T cells; indirect immunofluorescence: 3-4 μg/mL using paraformaldehyde fixed HeLa cells.; microarray: suitable; western blot: 0.25-0.5 μg/mL using cell extracts of PRMT6 transfected 293T cells
• UniProt accession no.: Q96LA8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRMT6(55170)

Description

General description

Protein arginine methyltransferases (PRMTs) are divided in two major types, type I (including PRMT1, 3, 4, and 6) and type II (PRMT5). Protein arginine methyltransferase 6 (PRMT6), a histone H3R2 methyltransferase encodes protein arginine methyltransferase 6. This gene is located on human chromosome 1p13.3.

Specificity

Anti PRMT6 (SE-15) antibody reacts specifically with PRMT6.

Immunogen

synthetic peptide corresponding to amino acids 2-16 of human PRMT6 conjugated to KLH via a C-terminal added cysteine.

Application

Anti-PRMT6 (SE-15) antibody produced in rabbit may be used in:

• western blotting
• immunofluorescence
• immunoprecipitation

Biochem/physiol Actions

Protein arginine methyltransferase 6 (PRMT6) enzyme is essential for the asymmetric dimethylation of H3R2 (H3R2me2a) especially at the arginine moiety. Overexpression of PRMT6 is seen in various human diseases including cancer. In MCF7 cells, deletion or blocking of this gene restores global DNA methylation. Absence of this gene results in the up-regulation of CDK-interacting protein 1 (p21 and p27) and cellular senescence. Antibodies specific for PRMT6 are an important tool for studying the biology of protein arginine methyltransferases (PRMTs).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)