P6361
Protease S from Pyrococcus furiosus
recombinant, expressed in Bacillus sp.
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About This Item
MDL number:
UNSPSC Code:
12352204
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recombinant
expressed in Bacillus sp.
form
solution
mol wt
45 kDa
concentration
100 units/mL
shipped in
wet ice
storage temp.
2-8°C
Application
Protease S is from Pyrococcus furiosus and is a recombinant protease that is expressed in Bacillus sp.. It is used for fragmentation of proteins and peptides required for primary structure analysis.
Biochem/physiol Actions
Protease S is a serine endoprotease with broad specificity that will digest native and denatured proteins. Cleavage occurs mainly on the carboxy side of peptide bonds. The optimal temperature range is 85 to 95 °C and the optimal pH range is 6.0 to 8.0. Protease S is inhibited by PMSF
Thermostable serine protease with broad specificity for native and denatured proteins.
Unit Definition
One unit will hydrolyze 1 μmol of N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide per minute at pH 7.0 at 95 °C.
Physical form
Solution in 25 mM Tris-HCl, pH 7.6, containing 40% ethanol.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
flash_point_f
78.8 °F
flash_point_c
26 °C
Certificates of Analysis (COA)
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