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P5746

Sigma-Aldrich

Monoclonal Anti-γ Parvin antibody produced in mouse

clone gparvin 40, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

gparvin 40, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

human, monkey, bovine

technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 4-8 μg/mL using total cell extracts of 293T cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PARVG(64098)

General description

Monoclonal Anti-γ Parvin (mouse IgG1 isotype) is derived from the gparvin 40 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. The parvin protein family belongs to the focal adhesion proteins. This family of proteins consists of three members α, β and γ parvin. γ parvin is expressed in lymphoid tissues. Parvin γ (PARVG) gene is located on human chromosome 22q13.31.

Specificity

Monoclonal Anti-γ Parvin recognizes human, monkey, and bovine γ parvin. It does not recognize mouse or rat γ parvin.

Immunogen

peptide corresponding to the C-terminal region of human γ parvin (amino acids 314-328).

Application

Monoclonal Anti-γ Parvin antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Monoclonal antibodies specific for γ parvin (PARVG) are an important tool for studying the biology of focal adhesion proteins. PARVG gene may have decreased expression in some cancer lines.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation
Johnstone CN, et al.
Molecular and Cellular Biology, 28(2), 687-704 (2008)
Parvin, a 42 kDa focal adhesion protein, related to the alpha-actinin superfamily
Olski TM, et al.
Journal of Cell Science, 114(3), 525-538 (2001)

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