The enzyme can be solubilized in 10 mM Tris-HCl, pH 7.4, containing 144 mM NaCl and 0.05% BSA. Store this product at –20 °C. The lyophilized product, when stored properly, retains activity for at least 2 years. A preparation of this enzyme in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0, and 10 mM EDTA retains activity at 2–8 °C for at least 3 years. Please see the link below to review additional product information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/218/p5542dat.pdf
P5542
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
lyophilized powder, ≥1,000 units/mg protein (Lowry)
Synonym(s):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
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All Photos(3)
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
form
lyophilized powder
Quality Level
specific activity
≥1,000 units/mg protein (Lowry)
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein
storage temp.
−20°C
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
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General description
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.[1]
Application
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane.[2][3] It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.[4]
Biochem/physiol Actions
Used for the release of GPI anchored proteins from the membrane.
Unit Definition
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Physical form
Contains phosphate buffer salts, EDTA and stabilizer
Analysis Note
Acetylcholinesterase is measured according to Ellman, et al.
inhibitor
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
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Which buffer should I use to reconstitute the enzyme in aqueous form and store at 4C or -20C?
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