P5412

o-Phenylenediamine

peroxidase substrate, chromogenic, tablet

• Synonym(s): 1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD
• Empirical Formula (Hill Notation): C₆H₈N₂
• CAS Number: 95-54-5
• Molecular Weight: 108.14
• Beilstein/REAXYS Number: 606074
• EC Number: 202-430-6
• MDL number: MFCD00007721
• UNSPSC Code: 12352204
• PubChem Substance ID: 24898638
• NACRES: NA.83

Properties

• product name: o-Phenylenediamine, tablet, 20 mg substrate per tablet
• vapor density: 3.7 (vs air)
• vapor pressure: 0.01 mmHg ( 25 °C)
• form: tablet
• bp: 256-258 °C
• mp: 100-102 °C
• fluorescence: λ_ex 337 nm; λ_em 417 nm (after derivatization)
• storage temp.: 2-8°C
• SMILES string: Nc1ccccc1N
• InChI: 1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
• InChI key: GEYOCULIXLDCMW-UHFFFAOYSA-N

Description

Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H₂SO₄ and read at 492 nm.

o-Phenylenediamine has been used to detect transforming growth factor (TGF-β) and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Safety Information

• pictograms: GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301,H317,H319,H341,H351,H410
• pcodes: P202 - P273 - P280 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: 276.8 °F - closed cup
• flash_point_c: 136 °C - closed cup