P4995

Anti-PRMT4 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CARM1, Anti-Protein-Arginine Methyl Transferase 4
• MDL number: MFCD05665250
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 5-20 μg using PRMT4-fusion protein from extracts of transfected 293T cells.; microarray: suitable; western blot: 0.5-1 μg/mL using nuclear extracts of the MCF7 breast carcinoma cell line.
• UniProt accession no.: Q86X55
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CARM1(10498) ; mouse ... Carm1(59035)

Description

General description

Protein arginine methyltransferase 4 (PRMT4), which is also known as coactivator associated arginine methyltransferase 1 (CARM1), is part of the protein arginine methyltransferase (PRMT) family. The gene encoding this protein is localized on human chromosome 19p13.2.

Immunogen

synthetic peptide corresponding to amino acids 48-63 of human PRMT4 conjugated to KLH via a C-terminal added lysine residue. The sequence is conserved in mouse.

Application

Anti-PRMT4 antibody produced in rabbit has been used for Western Blotting and immunofluorescence.

Biochem/physiol Actions

Protein arginine methyltransferase 4 (PRMT4) methylates arginine residues of histones and other proteins and thus has a role in modulation of gene expression. The protein also methylates histone acetyl transferases. It has been shown to associate with Mi2α, a chromatin remodeler. It also associates with nuclear factor κB (NF-κB), estrogen receptor and tumor suppressor p53 and is recruited to specific target genes. PRMT4 has a role in pre-mRNA splicing and DNA damage response. The protein has been shown to be upregulated in liver, prostate and breast cancers.

Protein-arginine methyl transferase 4 (PRMT4) plays a central role in upregulation of transcription by nuclear hormone receptors (NRs).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)