P4780

TWEEN^® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

• Synonym(s): POE (20) sorbitan monooleate, Polyethylene glycol sorbitan monooleate, Polyoxyethylenesorbitan monooleate, Polysorbate 80
• CAS Number: 9005-65-6
• MDL number: MFCD00082107
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• description: non-ionic
• form: viscous liquid
• mol wt: average mol wt ~1310
• composition: Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• CMC: 0.012 mM
• density: 1.06 g/mL at 25 °C (lit.); 1.07 g/cm³ at 25 °C (lit.)
• SMILES string: CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO
• InChI: 1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
• InChI key: RGPBUVUVZKQNHD-MDZDMXLPSA-N

Description

General description

TWEEN 80 is a polyethylene sorbitol ester and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.

TWEEN^® 80 has been used:

• in Listeria monocytogenes enumeration using filtration method
• as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion
• as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Gloves