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form
saline suspension
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
~50 μg/mL binding capacity (DNA)
storage temp.
2-8°C
Application
Protamine-agarose is used in protein chromatography, affinity chromatography and specialty resins. Protamine-agarose has been used to determine that Pichia pastoris (methylotrophic yeast) provides a convenient heterologous system for the production of recombinant subunits of human type 2A protein phosphatase (PP2A). Protamine-agarose has also been used to purify and characterize a novel protamine kinase in HL60 cells as well as to study myocardial infarction.
Physical form
Suspension in 0.5 M NaCl containing 0.02% thimerosal
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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I M Helander et al.
European journal of biochemistry, 163(1), 51-55 (1987-02-16)
The ability of agarose-linked protamine to bind Salmonella typhimurium lipopolysaccharides was investigated. Radioactively labelled lipopolysaccharides were isolated both from a smooth strain (SH6749, labelled with [14C]galactose) and from a rough strain (SH5014, lipopolysaccharide chemotype Rb2, labelled with [3H]acetate). From 50-micrograms
M Junco et al.
FEBS letters, 263(1), 169-171 (1990-04-09)
Protein kinase C (PKC) and its proteolysis-derived protein kinase independent of Ca2+ and phospholipids (PKM), were purified from rat brain. By using histone H1 and protamine as substrates, we assayed the effect of several inhibitors of PKC and PKM. The
S Shibata et al.
Journal of biochemistry, 112(4), 552-556 (1992-10-01)
Calphobindins (CPBs, placental annexins) are intracellular Ca(2+)- and phospholipid-dependent proteins like protein kinase C [EC 2.7.1.37]. We investigated the inhibitory effects of calphobindins on the protein kinase C activity in vitro. CPB I inhibited the protein kinase C activity for
V B Lokeshwar et al.
The Journal of biological chemistry, 264(32), 19318-19326 (1989-11-15)
Treatment of Swiss mouse 3T3 cells and human epidermoid carcinoma A431 cells with protamine at 37 degrees C increased the 125I-epidermal growth factor (EGF) binding activity at 4 degrees C. The effect of protamine on the increase of 125I-EGF binding
J L Rosales et al.
Journal of cellular physiology, 164(3), 509-521 (1995-09-01)
The role of ras on protein kinase C (PKC) signaling was examined in two keratinocyte cell lines. Increasing the level of extracellular calcium from 0.15 mM to 1.0 mM induces some features of differentiation in the spontaneously immortalized HaCaT line
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