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P3987

Sigma-Aldrich

Anti-Protein Kinase D antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PKCμ, Anti-PKD

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About This Item

MDL number:
MFCD03455823
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 110 kDa

species reactivity

human, mouse

technique(s)

immunoprecipitation (IP): 10 μL using cell lysate of mouse fibroblast NIH3T3 cell line.
microarray: suitable
western blot: 1:500 using whole cell extract of human epidermal carcinoma A431 cell line.

UniProt accession no.

Q15139

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PRKD1(5587)

General description

Protein Kinase D (also known as protein kinase C mu) is a protein that belongs to serine/ threonine (Ser/Thr) kinase family and is related to protein kinase C (PKC) family. It has a crucial role in maintaining Golgi structure and functions and can also act as a molecular switch for promoting cell proliferation and inhibiting apoptosis. Anti-Protein Kinase D antibody can be used in microarray and western blotting (diluted 1:500). Rabbit anti-protein kinase D antibody reacts specifically with PKD/PKC mu (110 kD) of mouse and human.

Immunogen

Synthetic peptide corresponding to the C-terminus of PKD/PKCmu (human, amino acids 893-912) conjugated to KLH.

Application

Anti-Protein Kinase D antibody can be used for detecting PKD/PKC mu by using several techniques like immunoprecipitation and immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Lot/Batch Number
061K4878

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C Jamora et al.
Cell, 98(1), 59-68 (1999-07-21)
We have shown previously that the betagamma subunits of the heterotrimeric G proteins regulate the organization of the pericentriolarly localized Golgi stacks. In this report, evidence is presented that the downstream target of Gbetagamma is protein kinase D (PKD), an

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