P3203

Anti-Protein Kinase Cβ₂ antibody produced in rabbit

0.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-PKC β₂
• MDL number: MFCD00162771
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen 80 kDa
• species reactivity: rat
• concentration: 0.5 mg/mL
• technique(s): dot blot: 1:1,000; microarray: suitable; western blot: 1:8,000 using rat brain extract
• UniProt accession no.: P68403
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Prkcb(25023)

Description

General description

Protein Kinase C (PKC, 76-93 kD) is a family of Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The conventional PKC isoforms (cPKC) are PKC-α, β1, β2 and γ; activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC ^{in vivo} is thought to be mediated by calpains I and II. Calpains cleave PKC in the V3 hinge region to produce two distinct fragments, one comprising the N-terminal regulatory domain (30 kD) and the other fragment containing the C-terminal kinase domain (50 kD) that is catalytically active. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C β2 antibody specifically recognises PKC β2 (80 kDa).

Specificity

In immunoblotting, the product reacts with PKC β₂ (80 kDa) using rat brain extract.

Immunogen

synthetic peptide corresponding to the C-terminal region of PKC β₂ (660-673).

Application

Anti-Protein Kinase C β2 antibody may be used for detection by immunoblotting at a working dilution of 1:8000 using rat brain extract. It may be used in dat blot at a working dilution of 1:1000. It is suitable for protein microarray.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified by affinity chromatography using immobilized PKC β₂ peptide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.