P3110

Anti-PDK1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD01634055
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 60 kDa
• species reactivity: human
• technique(s): microarray: suitable; western blot: 1:1,000 using recombinant human PDK1
• UniProt accession no.: O15530
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... PDPK1(5170)

Description

General description

The 3-phosphoinositide-dependent protein kinase-1 (PDK1, PKB kinase), is a master regulator of AGC kinases. It mediates cellular effects by activating major pathways involving PKB/Akt , S6K, RSK, SGK and the PKC isoforms. PDK1 is ubiquitously present in cells and is constitutively active. PDK1 is a critical activator of PKB/Akt as it mediates phosphorylation at Thr³⁰⁸ in the catalytic domain, thus plays a significant role on oncogenic signalling by PI3K, cell growth, survival, proliferation and migration. By regulating AGC kinases PDK1 is actively involved in the regulation of protein translation and metabolism, and serum and gluco-corticoid induced signalling.
Anti-PDK1 recognizes human PDK1 (60 kDa).

Immunogen

synthetic peptide corresponding to the C-terminus of human PDK1 (amino acids 538-556).

Application

Anti-PDK-1 may be used for detection by immunoblotting at a working dilution of 1:1000. The antibody is suitable for protein microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.