P3098
(−28) M13 Reverse primer set
Synonym(s):
5′-AGG AAA CAG CTA TGA CCA T-3′
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About This Item
UNSPSC Code:
41106305
Recommended Products
storage temp.
2-8°C
Application
(−28) M13 Reverse primer set is a single-stranded oligonucleotide with 5′-hydroxyl and 3′-hydroxyl ends and a selection of four fluorescent lables for use in polymerase chain reaction protocols (PCR).
Packaging
Packaged in amber tubes to protect from light.
Components
Set of four vials of primer, each individually labeled at the 5′-end with:
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl
FAM: (5[6]-carboxyfluorescein)
JOE: (6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein)
ROX: (5[6]-carboxy-X-rhodamine), and
TAMRA: (5[6]-carboxytetramethylrhodamine.
Supplied as solutions, 50 μl each, in tris-EDTA buffer, pH 7.5, at 1 picomole/μl
Analysis Note
Tested for purity with HPLC, PAGE, and OD.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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K A Abd-Elsalam et al.
Genetics and molecular research : GMR, 9(4), 2016-2024 (2010-10-20)
Seven fungal isolates were identified as pan-global Hypocrea/Trichoderma species, from section Trichoderma, on the basis of their morphology. These species were H. lixii/T. harzianum and H. orientalis/T. longibrachiatum. PCR-based markers with primer M13 (core sequence of phage M13) and internal-transcribed
Angela Capece et al.
International journal of food microbiology, 144(1), 187-192 (2010-10-12)
The present research studied Saccharomyces cerevisiae yeasts isolated from Nero d'Avola grapes, collected in different areas of the Sicily region. RAPD-PCR analysis with M13 primer was used for preliminary discrimination among 341 S. cerevisiae isolates. Inoculated fermentations with S. cerevisiae
High-throughput sequencing of PCR products tagged with universal primers using 454 life sciences systems.
Daigle D, Simen BB, Pochart P.
Current Protocols in Molecular Biology, Unit 7-Unit 7 (2011)
Yajing Zhou et al.
PloS one, 6(7), e22224-e22224 (2011-07-27)
Eukaryotic DNA polymerase δ (pol δ) plays a crucial role in chromosomal DNA replication and various DNA repair processes. It is thought to consist of p125, p66 (p68), p50 and p12 subunits. However, rigorous isolation of mammalian pol δ from
Jacquelina Williams-Woods et al.
Journal of virological methods, 178(1-2), 253-257 (2011-10-04)
Human norovirus (HuNoV) and hepatitis A (HAV) are recognized as leading causes of non-bacterial foodborne associated illnesses in the United States. DNA sequencing is generally considered the standard for accurate viral genotyping in support of epidemiological investigations. Due to the
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