P2996

Monoclonal Anti-PRMT6 antibody produced in mouse

clone PRMT6-74, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Protein-Arginine Methyl Transferase 6
• MDL number: MFCD05665251
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PRMT6-74, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using PRMT6 transfected 293T cell extracts
• isotype: IgG1
• UniProt accession no.: Q96LA8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRMT6(55170)

Description

General description

Monoclonal Anti-PRMT6 (mouse IgG1 isotype) is derived from the PRMT6-74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Protein arginine N-methyltransferase 6 (PRMT6) is a member of the type I PRMT enzyme family. This gene is located on human chromosome 1p13.3.

Specificity

Monoclonal Anti-PRMT6 (Protein-Arginine Methyl Transferase 6) recognizes human PRMT6 and does not cross react with PRMT1, 2, 3, 4, or 5

Immunogen

synthetic peptide corresponding to amino acids 358-373 of human PRMT6, conjugated to KLH.

Application

Monoclonal Anti-PRMT6 antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunoprecipitation

Biochem/physiol Actions

Arginine methylation is mediated by the protein arginine methyl transferase (PRMT) family of enzymes. Reduced PRMT6 expression is observed in melanoma. It is overexpressed in several cancers like, prostate, lung, cervix, breast and bladder. PRMT6 is known to control DNA methylation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)