P2992
Monoclonal Anti-p59fyn antibody produced in mouse
~1 mg/mL, clone 1S, purified immunoglobulin, buffered aqueous solution
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1S, monoclonal
form
buffered aqueous solution
species reactivity
human, rat, mouse
concentration
~1 mg/mL
technique(s)
capture ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-5 μg/mL using human lymphoma tissue
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FYN(2534)
mouse ... Fyn(14360)
rat ... Fyn(25150)
General description
The signalling in T cells is mediated by receptor and non-receptor protein tyrosine kinases (PTK). Two non-receptors PTK that belong to Src family, Fyn and Lck are vital for T-cell development and function. Both Fyn and Lck have unique domains that undergo post-translational modifications such as palmitoylation, myristoylation and acetylation od specific cysteine residues that are critical for T cell signalling. Fyn is generally associated with intracellular structures such as mitotic spindles. Both Fyn and Lck are closely associated in T cell differentiation, expansion and mediating TCR-mediated survival signals in naïve T cells. Both the kinases have partially overlapping functions and have the ability to compensate if either kinase is deficient.
Monoclonal Anti- p59fyn specifically recognizes human, mouse and rat Fyn protein (~59 kDa).
Monoclonal Anti- p59fyn specifically recognizes human, mouse and rat Fyn protein (~59 kDa).
Immunogen
amino acids 1-206 from recombinant murine p59fyn
Application
Monoclonal Anti- p59fyn may be used for detection by immunohistochemistry of formalin-fixed, paraffin-embedded human lymphoid tissue sections at a working concentration of 1-5 μg/mL. The recommended concentration for immunoprecipitation is 2μg/mg of lysate. The antibody is also suitable for immunoblotting and ELISA.
Physical form
Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dominik Filipp et al.
Molecular immunology, 41(6-7), 645-656 (2004-06-29)
While both lck and fyn are essential to generating the full sequelae of proximal signals emanating from the TcR/CD3 complex, thus far, modeling the temporal and spatial involvement of fyn has been problematic. The absence of a binding partner, analogous
A Wolven et al.
Molecular biology of the cell, 8(6), 1159-1173 (1997-06-01)
Members of the Src family of protein tyrosine kinases are localized to subspecialized regions of the plasma membrane. Herein we show that the N-terminal SH4 region of the Src family member p59fyn (Fyn) is both necessary and sufficient for targeting
M Koegl et al.
The Biochemical journal, 303 ( Pt 3), 749-753 (1994-11-01)
We have recently identified a novel N-terminal cysteine-containing motif which specifies the palmitoylation of several G-protein alpha-subunits [Parenti, Viganó, Newman, Milligan and Magee (1993) Biochem. J. 291, 349-353]. A related motif occurs at the N-terminus of members of the Src
S C Ley et al.
The Journal of cell biology, 125(3), 639-649 (1994-05-01)
Two src family kinases, lck and fyn, participate in the activation of T lymphocytes. Both of these protein tyrosine kinases are thought to function via their interaction with cell surface receptors. Thus, lck is associated with CD4, CD8, and Thy-1
Dominik Filipp et al.
The Journal of biological chemistry, 283(39), 26409-26422 (2008-07-29)
Mechanisms regulating the activation and delivery of function of Lck and Fyn are central to the generation of the most proximal signaling events emanating from the T cell antigen receptor (TcR) complex. Recent results demonstrate that lipid rafts (LR) segregate
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