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P2743

Sigma-Aldrich

Pyruvate Dehydrogenase Phosphatase from bovine kidney

buffered aqueous glycerol solution, ~850 units/mg protein

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About This Item

Enzyme Commission number:
3.1.3.43 (BRENDA, IUBMB)
MDL number:
MFCD04041079
UNSPSC Code:
12352204
NACRES:
NA.32

form

buffered aqueous glycerol solution

Quality Level

200

specific activity

~850 units/mg protein

UniProt accession no.

P35816

shipped in

dry ice

storage temp.

−70°C

Gene Information

cow ... PPM2C(280891)

General description

Pyruvate dehydrogenase phosphatase (PDP) (EC 3.1.3.43) contains two genetically and biochemically different isoforms, PDP1 and PDP2.[1] PDP1 is widely distributed and is composed of two subunits PDP1c, a catalytic subunit and PDP1r, a flavoprotein with a bound FAD.[2][3]

Biochem/physiol Actions

Insulin-activated Mg2+-dependent and Ca2+-stimulated mitochondrial protein phosphatase specific for the pyruvate dehydrogenase complex.
Pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1) catalyzes a Mg2+,-Ca2+ mediated dephosphorylation and activation of E1.[2] PDP1 plays a crucial role in the regulation of pyruvate dehydrogenase complex (PDC) activity.[1]

Unit Definition

One unit will releases 1 nmol of inorganic phosphate from 32P-labeled pyruvate dehydrogenase complex per min at 30 °C, pH 7.0.

Physical form

Solution in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-mercaptoethanol, 1 mM benzamidine, 0.1 mM PMSF, 1 mM EDTA, and 50% glycerol.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
071M1427
011M1436
040M1703
058K1066
057K1802

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Structural Requirements within the Lipoyl Domain for the Ca2+-dependent Binding and Activation of Pyruvate Dehydrogenase Phosphatase Isoform 1 or Its Catalytic Subunit
Turkan A, et al.
The Journal of Biological Chemistry, 277, 14976-14985 (2002)
Purification of Bovine Kidney and Heart Pyruvate Dehydrogenaseb Phosphatase on Sepharose Derivatized with the Pyruvate Dehydrogenase Complex
Pratt ML, et al.
European Journal of Biochemistry, 125, 349-355 (1982)
Down?regulation of Pyruvate Dehydrogenase Phosphatase in Obese Subjects is a Defect that Signals Insulin Resistance
Piccinini M, et al.
Obesity Research, 13, 678-686 (2005)

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