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P1143

Sigma-Aldrich

Pepsin from porcine gastric mucosa

Marker for denaturing PAGE

Synonym(s):

Pepsin A, Pepsin from hog stomach

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About This Item

CAS Number:
9001-75-6
EC Number:
3.4.23.1 (BRENDA, IUBMB)
EC Number:
232-629-3
MDL number:
MFCD00081840
UNSPSC Code:
12352200

form

powder

mol wt

35 kDa
 ~34.7 kDa

packaging

vial of 25 mg

color

white to light yellow

UniProt accession no.

P00791

Storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

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Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Recommended products

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
070H94591
095H9429
052H9461

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Benoît Stijlemans et al.
PLoS pathogens, 7(6), e1002072-e1002072 (2011-06-24)
The African trypanosome Trypanosoma brucei, which persists within the bloodstream of the mammalian host, has evolved potent mechanisms for immune evasion. Specifically, antigenic variation of the variant-specific surface glycoprotein (VSG) and a highly active endocytosis and recycling of the surface
Takahito Yuasa et al.
The American journal of pathology, 175(5), 1993-2003 (2009-10-10)
Wnt/beta-catenin signaling is required for skeletal development and organization and for function of the growth plate and articular cartilage. To further clarify these roles and their possible pathophysiological importance, we created a new transgenic mouse model in which Wnt/beta-catenin signaling
B I Hirschowitz
The Yale journal of biology and medicine, 72(2-3), 133-143 (2000-04-26)
Esophagitis results from excessive exposure of the esophagus to gastric juice through an ineffective or dysfunctional lower esophageal sphincter mechanism. A possible role of pepsin in damaging the esophageal mucosa with consequent esophagitis may be examined directly by testing pepsin
J Tack
Alimentary pharmacology & therapeutics, 24 Suppl 2, 10-16 (2006-08-31)
Gastro-oesophageal reflux disease is a multifaceted and multifactorial disorder which results from the reflux of gastric contents into the oesophagus. Animal studies suggest that synergism between acid and pepsin and conjugated bile acids have the greatest damaging potential for oesophageal
Hua-Nan Luo et al.
The Laryngoscope, 124(7), E294-E300 (2013-11-29)
To analyze the relationship between laryngopharyngeal reflux (LPR) represented by pepsin and pepsinogen, and pathogenesis of otitis media with effusion (OME). Prospective case-control study. Children with OME who required adenoidectomy and tympanostomy/tympanostomy tubes placement were enrolled in OME group, whereas
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