P0520
Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart
lyophilized powder (contains imidazole buffer salts and magnesium sulfate), >0.1 units/mg protein (with added activator), PI fraction of Ho, H.C., et al., Biochim. Biophys. Acta, 429, 461 (1976).
Synonym(s):
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE
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About This Item
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biological source
bovine heart
form
lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
specific activity
>0.1 units/mg protein (with added activator)
mol wt
~60 kDa
composition
Protein, ~75%
UniProt accession no.
foreign activity
5′-ATPase, alkaline phosphatase, and 2′:3′-cyclic nucleotide phosphodiesterase <0.005 units/mg protein
5′-nucleotidase <0.01 units/mg protein
inorganic pyrophosphatase <0.5 units/mg protein
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
Application
Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. [1] It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold. [2]
For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart. [3]
Packaging
Package size based on activated units
Unit Definition
One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.
Preparation Note
Crude preparation which has been stripped of the protein activator, calmodulin.
Reconstitution
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. If stored at 4 °C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.
Analysis Note
Protein determined by Warburg-Christian unless specified otherwise.
inhibitor
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.
R M Levin et al.
Molecular pharmacology, 13(4), 690-697 (1977-07-01)
N Seiler et al.
The international journal of biochemistry & cell biology, 30(3), 393-406 (1998-06-05)
N1-Dansylspermine and related sulfonamides of the natural polyamines are very potent blockers of NMDA-type glutamate receptors. They exhibit pharmacological properties which were not predicted from the constituents of the conjugates. Cytotoxicity and calmodulin antagonism of N1-dansylspermine were especially impressive. Calmodulin
P J Bekker et al.
Calcified tissue international, 51(4), 312-316 (1992-10-01)
Plasma membrane vesicles were prepared from chicken osteoclasts, and active calcium transport was demonstrated in a spectrofluorimetric assay using the fluorescent calcium concentration indicator, fura-2. Transport activity was inhibited by quercetin (10 microM), sodium vanadate (10 microM), and the anticalmodulin
C Y Huang et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(2), 871-874 (1981-02-01)
Kinetic studies on the activation of cyclic nucleotide phosphodiesterase (3',5'-cyclic-nucleotide 5'-nucleotidohydrolase, EC 3.1.4.17) as a function of calmodulin and Ca2+ concentrations have been carried out. A general approach to analyzing the mechanism of activation, which takes into consideration the various
Mark Roberge et al.
Toxicology letters, 154(1-2), 61-68 (2004-10-12)
Atrazine (ATR), 2-chloro-4-ethylamino-6-isopropylamino-s-triazine, has been implicated in numerous studies to act as an endocrine disruptor, specifically by altering estradiol signaling via increased aromatase activity. Fluorescence polarization (FP) was used to show that the binding equilibria between estrogen receptor-alpha or estrogen
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