The amount of powder in the vial will depend on the activity reported on the lot specific Certificate of Analysis. For example, lot 0000211383 is reported to have an activity of 115 units per milligram. A package of 1000 units would contain 8.7 mg. The 1000 unit package would prepare 870ul of a 10 mg/mL solution. See the link below to review this Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P0389-1KU0000211383.pdf
P0389
Penicillinase from Bacillus cereus
lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)
Synonym(s):
β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
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Select a Size
1000 UNITS
$72.36
5000 UNITS
$258.00
10000 UNITS
$401.20
25000 UNITS
$1,020.00
5 X 25 KU
$3,830.00
$72.36
List Price$76.90Save 6%In StockDetails
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1000 UNITS
$72.36
5000 UNITS
$258.00
10000 UNITS
$401.20
25000 UNITS
$1,020.00
5 X 25 KU
$3,830.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
$72.36
List Price$76.90Save 6%In StockDetails
Recommended Products
biological source
Bacillus sp. (Bacillus cereus)
Quality Level
form
lyophilized powder
specific activity
1,500-3,000 units/mg protein (using benzylpenicillin)
composition
Protein, ~10%
technique(s)
activity assay: suitable
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
2-8°C
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General description
Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis. [1]
Application
Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions. [2]
Used in the production of penicillin.
Biochem/physiol Actions
Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.
Unit Definition
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Physical form
Lyophilized powder containing phosphate and citrate buffer salts
Analysis Note
Protein determined by biuret.
inhibitor
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Properties of penicillinase from Bacillus cereus 569.
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An automated assay for the determination of penicillin in formulations suitable for use in pharmaceutical quality control is presented. The method is based on the classical iodometric penicillin assay which is incorporated in a flow injection analysis (FIA) system. The
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I have just received the product P0389-1KU 1000 units. The protocol I'm going to use requires me to use my 10 mg/ml solution. However, I don't understand what your "1000 Units" of product correspond to. Could you please give me more information pls?
1 answer-
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