P0294
Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle
For the Determination of ADP, buffered aqueous glycerol solution
Synonym(s):
PK/LDH enzymes from rabbit muscle
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5 ML
$186.76
5 X 5 ML
$758.00
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
$186.76
List Price$203.00Save 8%Web-Only Promotion
Available to ship onApril 29, 2025Details
Request a Bulk Order
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Quality Level
form
buffered aqueous glycerol solution
mol wt
59 kDa
concentration
600-1,000 units/mL pyruvate kinase
900-1400 units/mL lactic dehydrogenase
storage temp.
−20°C
General description
Pyruvate Kinase from rabbit muscle is a metalloenzyme which catalyzes the conversion of phosphoenol pyruvate to pyruvate in the glycolysis pathway. It corresponds to a molecular weight of 59 kDa.[1] It exists as a tetramer and undergoes conformational changes in the active site to accommodate substrate.[2] Lactic dehydrogenase (LDH) catalyzes the lactate to pyruvate conversion in anaerobic glycolysis. It exists as tetramer and comprises of two subunits (H and M).[3] The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality.[4]
Application
Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle has been used:
- for ATP generation in the active microtubule preparation[5]
- in the enzyme linked ATPase assay of skeletal muscle heavy meromyosin (HMM)[6]
- as a standard control for quantifying mesenchymal stem cells (MSCs) lactate dehydrogenase[7]
Biochem/physiol Actions
ADP Quantification Assay protocol for the use of PK/LDH in the determination of ADP. Solutions containing unkown concentrations of ADP can be substuted for reagent D in this protocol. Further dilutions of the ADP solution may be required
Lactate dehydrogenase from rabbit muscle can be inhibited by ascorbate. Aldolase and actin were shown to block this inhibitory effect. [8]
Pyruvate kinase also catalyzes the phosphorylation of thiamine diphosphate (TDP) to thiamine triphosphate (TTP) which may find application in antiviral and tumor therapy.[1]
Pyruvate kinase requires bivalent and monovalent cations such as Mg2+ and K+ respectively for activation to occur. [9]
Unit Definition
Pyruvate kinase activity: One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
Lactic dehydrogenase activity: One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Lactic dehydrogenase activity: One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Physical form
Solution in 50% glycerol containing 10 mM HEPES, pH 7.0, 100 mM KCl and 0.1 mM EDTA
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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