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P0249

Sigma-Aldrich

Anti-PAT (N-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Phosphinotricin Acetyl Transferase, Bar gene

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About This Item

MDL number:
MFCD08705493
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 21 kDa

species reactivity

plant

technique(s)

western blot: 1-2 μg/mL using extract (cytosolic fraction) of leves from transgenic Nicotiana tabacum expressing the PAT gene for Streptomyces hygroscopicus.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Specificity

The antibody recognizes bacterial PAT expressed in transgenic tobacco plants.

Immunogen

synthetic peptide corresponding to amino acids 1-17 located at the N-terminus of Streptomyces hygroscopicus phosphinotricin acetyl transferase (PAT), conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
095K4796

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Pawan Kumar Jewaria et al.
Plant & cell physiology, 54(8), 1253-1262 (2013-05-21)
The positioning and density of leaf stomata are regulated by three secretory peptides, EPIDERMAL PATTERNING FACTOR 1 (EPF1), EPF2 and stomagen. Several lines of published evidence have suggested a regulatory pathway as follows. EPF1 and EPF2 are perceived by receptor

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