P0104

Monoclonal Anti-PIASy antibody produced in mouse

~1.5 mg/mL, clone PIA4, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-PIAS4 (protein inhibitor of activated STAT, 4), Anti-Piasg
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PIA4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): immunocytochemistry: suitable; western blot: 1-2 μg/mL using total cell extract of HEK-293T cells transfected with human PIASy
• isotype: IgG1
• UniProt accession no.: Q8N2W9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PIAS4(51588)

Description

General description

Monoclonal Anti-PIASy (mouse IgG1 isotype) is derived from the hybridoma PIA4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Protein inhibitor of activated STAT4 (PIASy/PIAS4) belongs to the protein inhibitor of activated STAT (PIAS) family. It codes for a RING finger (RNF) protein. This gene is located on human chromosome 9p13.3.

Specificity

Monoclonal Anti-PIASγ recognizes human PIASγ.

Immunogen

synthetic peptide corresponding to amino acids 491-504 of human PIASy.

Application

Monoclonal Anti-PIASy antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

The protein inhibitor of activated STAT (PIAS) family of proteins are known to acts as small ubiquitin-related modifier (SUMO)−E3 ligase. PIASy can catalyze sumoylation of lymphoid enhancer factor 1 (LEF1). In addition, PIASy may have a direct role in the execution of the senescence program in cells. Thus PIASy has different regulatory mechanisms on different protein substrates. It serve as a negative modulator of innate immunity. PIAS4 is known to participate in ubiquitin signaling pathways. It has been found that sumoylation of p53 by PIASy regulates the interaction of p53 with MDM2 and drives the export of p53 from the nucleus to the cytoplasm.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)