P0096

Anti-POT1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-POT1-like telomere end-binding protein, Anti-PROTECTION OF TELOMERES 1, Anti-hPOT1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 55-58 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• technique(s): western blot: 1:1,000-1:2,000 using lysate of C2 or HeLa nuclear extract
• UniProt accession no.: Q9NUX5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... POT1(25913)

Description

General description

POT1 (Protection of Telomere 1, also known as POTE1 and POT1-like telomere end-binding protein) is a highly conserved single-strand telomeric binding protein. POT1 is one of the six core telomere-binding proteins termed the telosome2 or shelterin. POT1 has eukaryotic homologs and first identified in S. pombe. Mice that carry two POT1 genes encoding two proteins (POT1a and POT1b). Human genome contains one gene that encodes four alternative splice variants (v2, v3, v4, and v5).

Application

Anti-POT1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Protection of Telomere 1 (POT1) binds single-stranded telomeric DNA through two NH2-terminal oligonucleotide-binding folds and interacts with TTP1 through the COOH-terminal region. It is required for both chromosomal end protection and telomere length regulation. Genetic disruption of the two POT1 genes in mice revealed two different phenotypes. Knockout of Pot1a alone results in embryonic lethality. Pot1a/b double knockout cells showed enhanced telomere dysfunction induced foci.

Target description

POT1 (Protection of Telomere 1, also known as POTE1and POT1-like telomere end-binding protein) is a highly conserved single-strand telomeric binding protein and is required for both chromosomal end protection and telomere length regulation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)