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P0092

Sigma-Aldrich

Anti-PIR121 antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

ANTICytoplasmic FMR1 interacting protein 2 (CYFIP2), ANTIp53-inducible mRNA (PIR121)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~145 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 1-2 μg/mL using HEK-293T cells expressing human PIR121
western blot: 1-2 μg/mL using mouse brain extract

UniProt accession no.

Q96F07

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CYFIP2(26999)
mouse ... Cyfip2(76884)

General description

Cytoplasmic FMR1 interacting protein 2 (PIR121) (CYFIP2) is a vital constituent of the Wiskott−Aldrich Syndrome protein (WASp) −family verprolin−homologous protein (WAVE) regulatory complex. PIR121/CYFIP2 is a close homolog of CYFIP1 (p140Sra), with a sequence identity of 88%. The CYFIP2 gene is mapped to human chromosome 5q33.3

Specificity

Anti-PIR121 specifically recognizes human and mouse PIR121.

Application

Anti-PIR121 antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Cytoplasmic FMR1 interacting protein 2 (PIR121) (CYFIP2) is capable of interacting with fragile X mental retardation protein (FRMP) and also FRMP related proteins like, Fragile X related protein 1 and 2 (FXR1P and FXR2P). CYFIP2 helps to modulate spinal morphology and presynaptic activity. De novo hotspot variations in this gene is associated with early−onset epileptic encephalopathy.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
058K4828

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De novo hotspot variants in CYFIP2 cause early-onset epileptic encephalopathy
Nakashima M, et al.
Annals of Neurology, 83(4), 794-806 (2018)
A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P
Schenck A, et al.
Proceedings of the National Academy of Sciences, 98(15), 8844-8849 (2001)
Spatially clustering de novo variants in CYFIP2, encoding the cytoplasmic FMRP interacting protein 2, cause intellectual disability and seizures
Zweier M, et al.
European Journal of Human Genetics, 27(5), 747-759 (2019)
Daniel Humphreys et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(42), 16880-16885 (2013-10-03)
ADP ribosylation factor (Arf) 6 anchors to the plasma membrane, where it coordinates membrane trafficking and cytoskeleton remodelling, but how it assembles actin filaments is unknown. By reconstituting membrane-associated actin assembly mediated by the WASP family veroprolin homolog (WAVE) regulatory
Daniel Humphreys et al.
Cell reports, 17(3), 697-707 (2016-10-13)
To establish pathogenicity, bacteria must evade phagocytosis directed by remodeling of the actin cytoskeleton. We show that macrophages facilitate pathogen phagocytosis through actin polymerization mediated by the WAVE regulatory complex (WRC), small GTPases Arf and Rac1, and the Arf1 activator
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