P0045
Anti-p190-B RhoGAP (C-terminal region) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-ARHGAP5, Anti-GFI2, Anti-Growth factor independent 2, Anti-Rho GTPase activating protein 5, Anti-RhoGAP5
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~190 kDa
species reactivity
mouse, rat
packaging
antibody small pack of 25 μL
concentration
~1.5 mg/mL
technique(s)
western blot: 2-4 μg/mL using rat brain extract (S1 fraction) and a C2C12 lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ARHGAP5(394)
mouse ... Arhgap5(11855)
rat ... Arhgap5(299012)
General description
Rho GTPase activating protein 5 (ARHGAP5) or p190-B RhoGAP is a member of the RhoGTPase activating protein (RhoGAP family). ARHGAP5 gene is mapped to human chromosome 14q12 and is protumorigenic. p190-A and p190-B are the two isoforms that are highly expressed in the embryonic and adult brain.
Specificity
Anti-p190-B RhoGAP (C-terminal region) specifically recognizes rat and mouse p190-B RhoGAP.
Application
Anti-p190-B RhoGAP (C-terminal region) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Rho GTPase activating protein 5 (ARHGAP5) or p190-B RhoGAP functions as negative regulator of Rho activity. Deletion of p190-B in mice results in axonal-tract deficits and neuronal differentiation defects, indicating a central role of p190-B in normal brain development. Also, p190-B-deficient mice are reduced in size due to impaired insulin and insulin-like growth factors (IGF) signaling that affect adipogenesis. Several studies indicate a critical interaction between p190-B and IGF signaling pathway during embryonic mammary morphogenesis and epithelial progenitor cell migration. p190-B has shown to regulate the expression of membrane-type 1-matrix metalloproteinase (MT1-MMP) and matrix metalloproteinase 2 (MMP2) in endothelial cells and in modulating matrix remodeling and angiogenesis processes. It displays a elevated expression in hepatocellular cancer and promotes tumor spreading.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Yasuyuki Gen et al.
Cancer letters, 275(1), 27-34 (2008-11-11)
RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy
Fabien Guegan et al.
Journal of cell science, 121(Pt 12), 2054-2061 (2008-05-29)
The two isoforms of p190 RhoGAP (p190A and p190B) are important regulators of RhoGTPase activity in mammalian cells. Both proteins are ubiquitously expressed, are involved in the same signalling pathways and interact with the same identified binding partners. In search
Raffaella Sordella et al.
Cell, 113(2), 147-158 (2003-04-23)
Mature adipocytes and myocytes are derived from a common mesenchymal precursor. While IGF-1 promotes the differentiation of both cell types, the signaling pathways that specify the distinct cell fates are largely unknown. Here, we show that the Rho GTPase and
Brandy M Heckman et al.
Developmental biology, 309(1), 137-149 (2007-07-31)
P190-B RhoGAP (p190-B, also known as ARHGAP5) has been shown to play an essential role in invasion of the terminal end buds (TEBs) into the surrounding fat pad during mammary gland ductal morphogenesis. Here we report that embryos with a
Yuan Fang et al.
Oncotarget, 6(15), 13164-13175 (2015-05-12)
Nasopharyngeal carcinoma (NPC) is a highly invasive and metastasis-prone epithelial cancer. The paucity of effective treatment strategies for recurrent and metastatic NPC is the major cause for stagnating survival rate of NPC. Therefore, it's urgent to understand the molecular mechanisms
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