OGS595
PSF-RENILLA-PHOTINUS - BI-DIRECTIONAL FIREFLY RENILLA LUCIFERASE PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.85
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form
buffered aqueous solution
mol wt
size 6170 bp
bacteria selection
kanamycin
origin of replication
pUC (500 copies)
peptide cleavage
no cleavage
reporter gene
Renilla luciferase
shipped in
ambient
storage temp.
−20°C
General description
This plasmid has been designed so that sequences can inserted between two reporter genes (renilla and firefly luciferase) to allow the relative transcription level in both directions to be determined by measuring the reporter luminescence. Dual luciferase reporter assay kits are available from other providers that are compatible with this plasmid. The plasmid only contains a Bgl2 site between the two genes to allow insertion.
Promoter Expression Level:
Promoter Expression Level:
Application
This plasmid contains two luciferase reporter genes (Firefly and Renilla) in opposing directions with a Bgl2 restriction site in between the two genes to allow promoters to be inserted. This allows the transcriptional level of DNA sequences and promoters to be determined in both directions by inserting the sequences into the Bgl2 restriction site. If the sequence you would like to insert contains a Bgl2 site fragments with BamHI also have the same overhangs and can be ligated into this plasmid cut with Bgl2.
Sequence
To view sequence information for this product, please visit the product page
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
related product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Michael Craigie et al.
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JC virus (JCV) is a human polyomavirus and the etiologic agent of the demyelinating disease progressive multifocal leukoencephalopathy (PML). PML is observed in patients with underlying immunocompromising conditions, suggesting that neuro-immune interactions between peripheral immune cells and neuro-glia play an
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PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
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Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
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Carcinogenesis, 37(1), 18-29 (2015-10-28)
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