OGS244
PSF-CMV-UB-DAGFP - UBIQUITIN DAGFP PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC Code:
12352200
form
buffered aqueous solution
mol wt
size 5506 bp
bacteria selection
kanamycin
origin of replication
pUC (500 copies)
peptide cleavage
no cleavage
promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
This vector contains a green fluorescent protein called daGFP that is driven by the Ub promoter that is designed to be used as an add-on to any of our vectors. The Ub-daGFP expression cassette is flanked by AscI sites and is not designed to be split up. If you would like the daGFP gene alone or the Ub promoter alone please see the reporter gene section or the promoter section on our website. The Ub promoter is a relatively strong promoter. Its strength is approximately 10-fold lower than the CMV promoter but 10-fold stronger than the SV40 promoter. We have a weaker Rous Sarcoma virus (RSV) promoter driven vector in the same format if you require an expression vector with lower levels of reporter gene activity.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Application
Each restriction site in this vector has been positioned for a pre-designed purpose and allows the insertion of specific DNA sequences from our other DNA plasmids. To insert a gene we recommend using any sites between (and including) the HindIII and XbaI sites. By doing this you will be able to insert most of our other products upstream or downstream of your gene.
The BsgI and BseRI restriction sites in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here.
The BsgI and BseRI restriction sites in the plamsid cleave within the stop codon in the XbaI site in the MCS. This can allow retrospective fusion of coding sequences to our peptide tag range if your genes stop codon is positioned here.
Sequence
Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Genebank Vector Sequence File
FASTA Vector Sequence File
Full Plasmid Map
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.
Other Notes
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
Legal Information
Oxford Genetics is a trademark of Oxford Genetics Ltd
related product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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