OGS185
PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.85
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form
buffered aqueous solution
mol wt
size 4378 bp
bacteria selection
kanamycin
origin of replication
pUC (500 copies)
peptide cleavage
no cleavage
promoter
Promoter name: T7
Promoter activity: inducible
Promoter type: phage
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID allows transcription of un-polyadenylated and un-capped RNA using the T7 bacteriophage polymerase. This plasmid also contains the internal ribosome entry site (IRES) from Encephalomyocarditis virus (ECMV) between the promoter and the multiple cloning site (MCS). It is designed to be used to express genes using eukaryotic T7 expression systems such as those used for the recovery of a variety of viruses. This vector is not designed for the expression of two proteins from the same mRNA. For this purpose we have designed pSF-CMV-MCS-IRES-PciI where the PciI site contains a start codon in the correct position for the IRES to express efficiently. In this vector there is an NcoI site (which contains an ATG start codon) in the correct position to enable efficient expression of a gene in the MCS.
application
The start codon that is within the NcoI restriction site is positioned to allow efficient expression from the ECMV IRES. The main MCS for gene insertions in this molecular cloning vector therefore extends from NcoI to XbaI. Downstream sites have alternate functions and are used to insert other DNA sequences that we sell however they can be used to insert a gene if these functions are not required.
The ClaI to NheI sites have other functions such as adding peptide tags second promoters or other IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.
The ClaI to NheI sites have other functions such as adding peptide tags second promoters or other IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.
Sequence
To view sequence information for this product, please visit the product page
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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