O9635

Oncostatin M human

BioReagent, ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): OSM
• CAS Number: 106956-32-5
• EC Number: 200-835-2
• MDL number: MFCD00166731
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• product line: BioReagent
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤1.000 ng/mL ED₅₀
• mol wt: ~22 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• solubility: soluble 0.100 mL, clear, colorless (Solvent-PBS + 0.1% BSA)
• UniProt accession no.: P13725
• storage temp.: −20°C
• Gene Information: human ... OSM(5008)

Description

General description

Oncostatin M (OSM) is produced by activated T cells, monocytes and Kaposi′s sarcoma cells. OSM shares several structural and functional characteristics with leukemia inhibitory factor (LIF), interleukin-6 (IL-6), and ciliary neurotrophic factor (CNTF).

Application

Human oncostatin-M has been used to stimulate bovine full-depth cartilage explants. It has also been used to culture embryonic stem cells in standard minimum essential medium-α.

Biochem/physiol Actions

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. It can exert both stimulatory and inhibitory effects on cell proliferation. OSM stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi′s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release [e.g. interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte-colony-stimulating factor (G-CSF)] from endothelial cells.

Oncostatin M (OSM), LIF, G-CSF, IL-6, and CNTF are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. OSM is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. OSM is secreted by macrophages and activated T lymphocytes.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid, containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The proliferative activity of human oncostatin M is tested in culture by using a human erythroleukemic cell line, TF-1.

Safety Information

• Storage Class: 4.1B - Flammable solid hazardous materials
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter